Freezing Injury and Resistance in Spinach Chloroplast Grana

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Freezing injury and resistance in spinach chloroplast grana.

Spinach grana appear to be injured by the same mechanism and by the same degree of dehydration and volume reduction that injures animal cells. Winter-hardened or artificially protected grana avoid injury by permitting a reversible influx of solute which forestalls excessive dehydration and shrinkage.

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Carotenoid composition of spinach chloroplast grana and stroma lamellae.

Stroma lamellae and grana stacks prepared by French press rupture of spinach (Spinacia oleracea) chloroplasts contain similar amounts of beta-carotene on a protein basis. The grana fraction has considerably more xanthophylls than does the stroma fraction. Total carotenoid to chlorophyll ratios are similar for both fractions.

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Biosynthesis of grana and stroma lamellae in spinach.

Park and co-workers (Annu. Rev. Plant Physiol. 22: 395-430) have suggested that stroma lamellae, which perform only photosystem I contain a developing photosystem II which becomes functional upon the folding of these membranes to form grana stacks.The present study was undertaken to test this hypothesis. The lipid and protein constituents of chloroplast membranes were pulse-labeled radioactivel...

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Chloroplast replication and chloroplast DNA synthesis in spinach leaves

Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli­ cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...

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Chloroplast replication and chloroplast DNA synthesis in spinach leaves

Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and repli­ cation. Autoradiographs prepared from thin sections show th a t labelling throughout disks is uniform. We consider th a t cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinac...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1970

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.45.6.752